Characterization of the Bacillus subtilis CwbA protein which stimulates cell wall lytic amidases.

نویسندگان

  • A Kuroda
  • J Sekiguchi
چکیده

The Bacillus subtilis cell wall binding protein, CwbA, stimulated the cell wall lytic activities of the B. subtilis and B. licheniformis autolysins (CwlA and CwlM, respectively) in addition to that of the major B. subtilis autolysin (CwlB). Even though the substrate for the enzyme reaction was changed from B. subtilis cell wall containing a teichoic acid to Micrococcus luteus cell wall containing a teichuronic acid, the stimulatory effect of CwbA on CwlA activity was observed.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Expression of a germination-specific amidase, SleB, of Bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores.

A germination-specific amidase of bacilli is a major spore-lytic enzyme that is synthesized with a putative signal sequence and hydrolyses spore cortex in situ. The sleB gene encoding this amidase in Bacillus subtilis and Bacillus cereus was expressed in the forespore compartment of sporulating cells under the control of sigmaG, as shown by Northern blot and primer extension analyses. The fores...

متن کامل

Binding site-shaped repeated sequences of bacterial wall peptidoglycan hydrolases.

The non-catalytic C-terminal regions of the N-acetylmuramidase (lysozyme) of Clostridium acetobutylicum and N-acetylmuramoyl(D-lactyl)-L-alanine amidases CwlA of Bacillus subtilis, ORFL3 and CwlL of Bacillus licheniformis were previously reported to have similarities with the amino acid sequence of the non-catalytic N-terminal module of the Streptomyces albus G Zn DD-peptidase. This peptidase i...

متن کامل

The lytE gene of Bacillus subtilis 168 encodes a cell wall hydrolase.

Bacillus subtilis cell wall-bound protein CWBP33 is encoded by lytE, a gene expressed during the exponential growth phase. Sequence analysis of LytE, a 33-kDa protein, reveals two domains. The N-terminal domain contains a threefold-repeated motif common to several peptidoglycan binding proteins, while the C-terminal domain, probably carrying the catalytic activity, has homology with certain exo...

متن کامل

Biochemical Aspects of Competence in the Bacillus Subtilis Transformation System. Ii. Autolytic Enzyme Activity of Cell Walls.

A lytic factor, associated with the cell wall, was fortuitously discovered during the course of the investigations described in the previous paper (1). In the pneumococcal transformation system (2-5) as well as in the Bacillus subtilis transformation system (6), the highly transformable strains possessed active autolytic enzymes. Consequently, studies were undertaken to investigate the relation...

متن کامل

Biochemical Aspects of Competence in the Bacillus subtilis Transformation System

A lytic factor, associated with the cell wall, was fortuitously discovered during the course of the investigations described in the previous paper (1). In the pneumococcal transformation system (2-5) as well as in the Bacillus subtilis transformation system (6), the highly transformable strains possessed active autolytic enzymes. Consequently, studies were undertaken to investigate the relation...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • FEMS microbiology letters

دوره 74 1  شماره 

صفحات  -

تاریخ انتشار 1992